Immunochemical techniques laboratory manual /

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Bibliographic Details
Main Author: Goers, John
Format: Book
Language:English
Published: San Diego : Academic Press, c1993.
Subjects:
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Table of contents
Table of Contents:
  • Machine derived contents note: Table of contents for Immunochemical techniques laboratory manual / John Goers.
  • Bibliographic record and links to related information available from the Library of Congress catalog
  • Information from electronic data provided by the publisher. May be incomplete or contain other coding.
  • Methods Locator.
  • Preface.
  • Notes to Students and Instructors :
  • Student Background for Laboratory, Immunochemistry Supplies, Antibody Requirements for Experiments, Suggested Schedule of Experiments.
  • Background: Review of the Immune System and Immunoglobulin Structure.
  • Experiments:
  • Preparation of Serum from Blood.
  • Purification and Analysis of IgG.
  • Quantitative Precipitin Curve.
  • Precipitation Analysis in Gel.
  • Affinity Chromatography of Antibodies.
  • Labeling Antibodies.
  • Protein Blotting.
  • Immunoprecipitation with Staphylococcal Protein A.
  • Immunocytochemical Staining of Lymphocytes.
  • Enzyme-Linked Immunosorbent Assay: ELISA. Appendices: Chemistries and Procedures for Anninity Chromatography.
  • Buffers.
  • PAGE Reagent and Staining Procedures.
  • Western Blotting Reagents and Staining Procedures.
  • Suppliers of Equipment and Chemical Reagents.
  • Saturated Ammonium Sulfate Table.
  • Radioiodination of Proteins.
  • Immunization Procedure.
  • Detergent Solubilization of Proteins.
  • Index.
  • Library of Congress subject headings for this publication: Immunochemistry Laboratory manuals, Immunoassay Laboratory manuals, Immunoassay methods, Immunochemistry laboratory manuals.